![]() Example: Suppose you diluted 2 liters of juice with 3 liters of water, calculate the dilution factor. The results I'm getting are too low though, and i'm afraid that i'm not taking into account the dilution correctly. how to calculate the concentration of the diluted solutions if they unknown First: find the D.F: Dilution factor (D.F) final volume / aliquot volume. Dilution factor is the ratio of the solute to solvent or simply the ratio of the volume of the initial solution to the volume of the final solution. Convert a ratio into the exact amount of product and water required. I want to measure the amount of HCP in in the 3 peaks, so what i did and I pooled the fractions together (for each peak) and used an assay to measure HCP. Make calculating dilution ratios easy with Clover Chemicals Dilution Ratio Calculator. ![]() I'm collecting 2ml fractions, so I get 15 fractions for the FT, 7 for the Eluate and 1 for the Strip. ![]() I get a wide FT flat peak, an elution peak and a tiny peak at the end (strip). I inject 30ml into the 1ml column (at 1ml/min). I take my harvest and dilute it 1:4 in buffer A. i think it's a pretty simple problem but i'm not sure my logic is correct.īackground info: CHO harvest feed is injected onto a 1ml AIEX column using an Akta avant. I have a question about calculating concentration of HCP in fractions collected from an AIEX run and I can't get my head around it.
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